HPLC (Size Exclusion) Analysis of Proteins on Zenix^®-C SEC-300, Effect of DTT and EDTA

技术

HPLC: suitable

试验参数

column: Zenix-C SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777092)
mobile phase: (A) 150 mM sodium phosphate buffer, pH 7.0 (B) 150 mM sodium phosphate buffer, pH 7.0 with 2 mM DTT, 0.1 mM EDTA (C) 150 mM sodium phosphate buffer, pH 7.0 with 2 mM DTT, 0.1 mM EDTA
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 280 nm
injection: 10 μL
sample: proteins

适用性

application for HPLC

应用

life science and biopharma
pharmaceutical (small molecule)