HPLC Analysis of a Peptide Drug on Antibodix^® WCX-NP10, Gradient Optimization to Separate Impurity

技术

HPLC: suitable

试验参数

column: Antibodix WCX-NP10, 25 cm x 4.6 mm I.D., 10 μm particles (Z777272)
mobile phase: [A] 3 mM potassium phosphate, pH 5.0; [B] 20 mM potassium phosphate, pH 7.5
gradient: (A) 0% - 50% B (20 min); (B) 0% - 20% B (20 min), 20% B after 20 min
flow rate: 1 mL/min
column temp.: ambient
detector:
injection: 5 μL
sample: 2 mg/mL, proprietary peptide drug, pI 7-8

适用性

application for HPLC

应用

life science and biopharma
pharmaceutical (small molecule)