技术
HPLC: suitable
试验参数
column: Ascentis Express OH-5, 15 cm x 4.6 mm, I.D., 2.7 μm particles (53778-U)
mobile phase: [A] 0.005 M ammonium formate, pH 3.15 with concentrated TFA [B] acetonitrile (30:70, A:B)
flow rate: 0.5 mL/min
pressure: 841 psi (58 bar)
column temp.: 35 °C
detector: ELSD (detector other: Nebulizer 60º C, Evaporator 80º C, Gas flow 1.60 SLM (Standard Liters/ Minute), LED 100%, Smoothing 5.0 S, PMT ( Photomultiplier) Gain 1.0)
injection: 10 μL
sample: 0.5 mg/mL each prepared in 50:50 Water:Acetonitrile
适用性
application for HPLC
应用
food and beverages
分析说明
This application shows the analysis of sugars by HILIC Chromatography with ELSD detection. The purpose of the work shown here was to develop a HILIC method that could quickly separate mono- and di-saccharides with good sensitivity and reproducibility utilizing the Ascentis Express OH-5 column and an Evaporative Light Scattering Detecctor (ELSD) detector.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Dextrose, Pharmaceutical Secondary Standard; Certified Reference Material
Sucrose, Pharmaceutical Secondary Standard; Certified Reference Material
Maltitol, Pharmaceutical Secondary Standard; Certified Reference Material
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