技术
HPLC: suitable
试验参数
column: Zenix SEC-300, 30 cm X 4.6 mm I.D., 3 μm particles (Z777087)
mobile phase: 0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide
flow rate: 0.35 mL/min
pressure: 1350 psi (93 bar)
column temp.: ambient
detector: UV, 215 nm
injection: 1 μL
sample: digested SO57 mAb, ~0.5 g/L
适用性
application for HPLC
应用
life science and biopharma
分析说明
Proteolysis of antibodies can be useful for simplifying further analysis. This application demonstrates separation of the two fragments, F(ab′)2 and scFc from the intact IgG, after partial digestion with IdeS protease.
法律信息
Zenix is a registered trademark of Sepax Technologies
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