技术
HPLC: suitable
试验参数
column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase: 2 mM ammonium formate (90:10 acetonitrile:water) adjusted to pH 4.5 with formic acid
flow rate: 0.4 mL/min
column temp.: 35 °C
detector: ESI(+), TIC 100 – 1000 m/z; APCI (+), XIC m/z = 150.1, 203.7, 378.2
injection: 1 μL
sample: 10 μg/mL in 75:25 acetonitrile:water
适用性
application for HPLC
应用
forensics and toxicology
分析说明
Dermorphin is a heptapeptide derived from frogs. It is a painkiller and used illicitly in racehorses so they continue to run through the pain. A rapid analysis of dermorphin standard is shown here on an Ascentis Express F5 column. The HILIC mode separation provided the necessary retention and MS-compatibility, while the pentafluorophenyl (F5) phase chemistry elimated the chelating interactions that are problematic on bare silica.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Dermorphin trifluoroacetate salt, ≥98% (HPLC)
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门