技术
HPLC: suitable
试验参数
column: Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 μm particles (53929-U)
mobile phase: [A] water with 0.1% TFA; [B] acetonitrile with 0.1% TFA; (65:35, A:B)
flow rate: 1 mL/min
pressure: 2200 psi (152 bar)
column temp.: 30 °C
detector: UV, 250 nm
injection: 5 μL
sample: 50 μg/mL in 75:25, water:acetonitrile
适用性
application for HPLC
应用
pharmaceutical (small molecule)
分析说明
Salicylic acid is the primary metabolite of aspirin, and is likely responsible for its antiinflammatory properties via suppression of cyclooxygenase genes; it also is thought to suppress colonic carcinogenesis. Ascentis Express RP-Amide provides good retention and resolution at low pH by a combination of hydrophobic and H-bonding intereactions.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Acetylsalicylic acid, analytical standard
Salicylic acid, BioXtra, ≥99.0%
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