技术
HPLC: suitable
试验参数
column: Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U)
mobile phase: [A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C)
flow rate: 0.6 mL/min
pressure: 812 psi (56 bar)
column temp.: 35 °C
detector: ELSD, 55 °C, 3.5 bar nitrogen
injection: 2 μL
sample: dilute1:9 in acetonitrile
适用性
application for HPLC
应用
food and beverages
分析说明
This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UV and ELSD detectors in series, this chromatogram being from the ELSD.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Sucrose
Taurine
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