LC/MS Analysis of Sertraline and Norsertraline on Ascentis^® Express RP-Amide

技术

HPLC: suitable

试验参数

column: Ascentis Express RP Amide, 10 cm x 3.0 mm I.D., 2.7 μm particles (53918-U)
mobile phase: [A] 10 mM ammonium formate, water, pH 3.6; [B] 10 mM ammonium formate, acetonitrile, pH 3.6; (65:35, A:B)
flow rate: 0.6 mL/min
column temp.: 55 °C
detector: ESI(+), 100-1000 m/z
injection: 2 μL
sample: 200 ng/mL in 25:75, water: acetonitrile

适用性

application for HPLC

应用

clinical
pharmaceutical (small molecule)