LC/MS Analysis of Polar Amino Acids on Ascentis^® Si (HILIC Mode)

技术

HPLC: suitable

试验参数

column: Ascentis Si, 10 cm x 2.1 mm I.D., 5 μm particles (581508-U)
mobile phase: [A] 100 mM ammonium formate, pH 3.0 with formic acid; [B] water; [C] acetonitrile
gradient: 5% A constant; 10 to 16% B, 85 to 79% C in 20 min
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: ESI(+)
injection: 2 μL
sample: 0.1 g/L each in mobile phase

适用性

application for HPLC

应用

life science and biopharma