LC/MS Analysis of Omeprazole and Metabolites on Ascentis^® Express RP Amide (pH 3.4)

技术

HPLC: suitable

试验参数

column: Ascentis Express RP-Amide, 5 cm x 2.1 mm I.D., 2.7 μm particles (53911-U)
mobile phase: [A] 10 mM ammonium formate in water, pH 3.4; [B] 10 mM ammonium formate in methanol, pH 3.4; (65:35, A:B)
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: ESI(+), m/z 50-400
injection: 1 μL
sample: 1 mg/L in mobile phase

适用性

application for HPLC

应用

clinical