跳转至内容
Merck
CN
所有图片(1)

文件

G005711

LC/MS Analysis of Omeprazole and Metabolites in Plasma on Ascentis® Express RP Amide after Sample Prep using HybridSPE®-Phospholipid

application for HPLC, application for SPE

登录查看公司和协议定价
所有图片(1)

技术

HPLC: suitable
solid phase extraction (SPE): suitable

试验参数

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: rat plasma spiked with omeprazole, phenacetin and metabolites at 2 μg/mL
SPE well plate: HybridSPE-Phospholipid, 96-well plate, 15 mg/0.8 mL (575656-U)
sample addition: 100 μL plasma to each well, followed by 300 mL of 1% formic acid in acetonitrile
elution: apply vacuum
eluate post-treatment: eluate was collected, evaporated, and reconstituted in mobile phase.
column: Ascentis Express RP-Amide, 5 cm x 2.1 mm I.D., 2.7 μm particles (53911-U)
mobile phase: [A] water, 10 mM ammonium formate, pH 3.4; [B] methanol, 10 mM ammonium formate, pH 3.4; (65:35, A:B)
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: ESI(+), m/z = 50-400
injection: 1 μL

适用性

application for HPLC
application for SPE

应用

clinical

应用

This application demonstrates the suitability of HybridSPE and the Ascentis Express RP Amide for the analysis of omeprazole metabolites.

分析说明

Sample was vorlexed and centrifuged for 2 minutes at 15000 rpm. Supemate was then passed through HybridSPE 96 well plate. Elutent was collected, evaporated and reconstituted in mobile phase.

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

分析物

说明
    5-Hydroxyomeprazole
    Phenacetin
    Omeprazole
    Omeprazole sulfone

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门