LC/MS Analysis of Omeprazole and Metabolites on Ascentis^® Express HILIC

技术

HPLC: suitable

试验参数

column: Ascentis Express HILlC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)
mobile phase: [A] 5 mM ammonium formate in water, pH 6.5; [B] 5 mM ammonium formate in acetonitrile, pH 6.5; (2:98, A:B)
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: ESI(+), m/z 50-400
injection: 0.1 μL
sample: 1 mg/L each in mobile phase

适用性

application for HPLC

应用

clinical