技术
HPLC: suitable
试验参数
column: Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)
mobile phase: [A] 100 mM ammonium formate, pH 3.0 (titrated with formic acid); [B] acetonitrile; (10:90, A:B)
flow rate: 1.0 mL/min
pressure: 1247 psi (86 bar)
column temp.: 35 °C
detector: ESI(+); SIR
injection: 5 μL
适用性
application for HPLC
应用
pharmaceutical (small molecule)
分析说明
This application shows the suitability of Ascentis Express Silica columns for the analysis of erythromycin analogs in aqueous normal phase/HILIC mode of chromatography. Other relatively hydrophobic aminoglycosides have also been shown to separate under these conditions.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Erythromycin ethyl succinate
Erythromycin estolate
Erythromycin
分析色谱柱
标准
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