技术
HPLC: suitable
试验参数
column: Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)
mobile phase: [A] 20 mM ammonium acetate, pH 4.7 with acetic acid: [B] acetonitrile; (90:10, A:B)
flow rate: 0.6 mL/min
column temp.: 40 °C
detector: UV, 214 nm
injection: 1 μL
sample: 100 - 500 μg/mL in 95:5, buffer: acetonitrile
适用性
application for HPLC
应用
food and beverages
分析说明
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of common drink additives used in diet soda.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Caffeine
Benzoic acid
Asp-Phe methyl ester
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