HPLC Analysis of Tryptic Digest of Protein Mixture on BIOshell A160 2.7 μm Peptide C18

技术

HPLC: suitable

试验参数

column: BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)
mobile phase: [A] 0.1% formic acid in water; [B] 0.4% formic acid in 25:75 (v/v) water:acetonitrile
gradient: 6 to 70% B in 40 min
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: ESI(+), TOF
injection: 2 μL
sample: 10 pmol/L in 0.1% formic acid

适用性

application for HPLC

应用

life science and biopharma