LC/MS Analysis of Vaccinium Anthocyanidins on Ascentis^® Express C18 at Elevated Temperature and Flow

技术

HPLC: suitable

试验参数

column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase: [A] 0.1% formic acid in water; [B] 0.1% formic acid in 75:25 (v/v) acetonitrile:water; (78:22, A:B)
flow rate: 0.6 mL/min
pressure: 5440 psi (375 bar)
column temp.: 35 °C
detector: ESI(+), TOF
injection: 1 μL
sample: 20 μg/mL in 0.1% formic acid

适用性

application for HPLC

应用

vitamins, nutraceuticals, and natural products