技术
HPLC: suitable
试验参数
column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase: [A] water; [B] acetonitrile
gradient: 20 to 100% B in 5 min; held at 100% B for 0.5 min
flow rate: 0.3 mL/min
pressure: 2750 psi (190 bar)
column temp.: 35 °C
detector: UV, 227 nm
injection: 5 μL
sample: 20 mg/mL in water:acetonitrile (50:50); sonicate for 15 minutes; filter 0.45 μm; dilute to water:acetonitrile (80:20)
适用性
application for HPLC
应用
vitamins, nutraceuticals, and natural products
分析说明
This application demonstrates the suitability of Ascentis Express F5 for the efficient separation of withania extract.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Withanoside IV
Withanoside V
Withaferin A
12-Deoxywithastramonolide
Withanolide A
Withanone
Withanolide B
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