GC Analysis of Volatiles in Roasted Arabica Coffee on SUPELCOWAX 10 after SPME using 50/30 μm DVB/Carboxen/PDMS Fiber

技术

gas chromatography (GC): suitable
solid phase microextraction (SPME): suitable

试验参数

sample/matrix: Arabica coffee beans, roasted, 2 grams with pre-equilibration of 10 min at 60 °C, the entire process under agitation at 500 rpm
SPME fiber: DVB/Carboxen/PD, 50/30 μm, Stableflex (57328-U)
extraction: headspace, 40 min at 60 °C
desorption process: 5 min
column: SUPELCOWAX 10, 30 m × 0.25 mm I.D., 0.25 μm (24079)
oven: 40 °C (5 min), 4 °C/min to 230 °C, 50 °C/min to 280°C (2 min)
inj. temp.: 260 °C
detector: MS (Shimadzu GCMS-QP2010) ionization energy 1.5 kV; mass range: 40-400 amu; scan mode, scan interval : 0.5 sec
MSD interface: 230 °C, source temp. 200 °C
carrier gas: helium, 34.0 cm/s and 40.3 KPa
injection: splitless 5 min, then split ratio 1:20
liner: 0.75 mm i.d.

适用性

application for SPME

应用

food and beverages