HPLC Analysis of Withania (Aswagandha) Standard on Ascentis^® Express F5

技术

HPLC: suitable

试验参数

column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase: [A] water; [B] acetonitrile
gradient: 20 to 100% B in 10 min: held at 100% B for 0.5 min
flow rate: 0.3 mL/min
pressure: 2756 psi (190 bar)
column temp.: 35 °C
detector: UV, 227 nm
injection: 5 μL
sample: 20 μg/mL in 80:20, water:methanol

适用性

application for HPLC

应用

vitamins, nutraceuticals, and natural products