技术
HPLC: suitable
试验参数
column: Ascentis Express C18, 10 cm x 3 mm I.D., 2.7 μm particles (53814-U)
mobile phase: [A] water with 0.1% formic acid; [B] methanol
gradient: held at 35% B for 3 min; 35 to 45% B in 10 min; held at 45% B for 2 min; 45 to 100% B in 5 min
flow rate: 0.6 mL/min
pressure: 4105 psi (283 bar)
column temp.: 35 °C
detector: UV, 254 nm
injection: 20 μL
sample: 20 μg/mL in 80:20, water:methanol
适用性
application for HPLC
应用
vitamins, nutraceuticals, and natural products
分析说明
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of milk thistle related compounds.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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