HPLC Analysis of Pilocarpine, Betaxolol, and Benzalkonium Chloride on Ascentis^® Express HILIC

技术

HPLC: suitable

试验参数

column: Ascentis Express HILIC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)
mobile phase: [A] 20 mM ammonium acetate; [B] acetonitrile; (10:90, A:B)
flow rate: 1 mL/min
column temp.: 35 °C
detector: ESI (+), XIC m/z 304, 332 (BAC), 209 (pilocarpine), 308 (betaxolol)
injection: 2 μL
sample: 10 mg/L in acetonitrile

适用性

application for HPLC

应用

pharmaceutical (small molecule)