HPLC Analysis of Norphenylephrine Enantiomers on Astec^® CYCLOBOND I 2000 AC (pH 5.0)

技术

HPLC: suitable

试验参数

column: CYCLOBOND I 2000 AC, 25 cm x 4.6 mm I.D., 5 μm particles (20124AST)
mobile phase: [A] methanol; [B] 20 mM ammonium acetate, pH 5.0; (5:95, A:B)
flow rate: 0.5 mL/min
column temp.: 25 °C
detector: UV, 230 nm
injection: 5 μL
sample: norphenylephrine, 1 mg/mL in acetonitrile:water (50:50)

适用性

application for HPLC

应用

pharmaceutical (small molecule)