HPLC Analysis of cis/trans-Dothiepin Enantiomers on Astec^® CYCLOBOND I 2000 AC

技术

HPLC: suitable

试验参数

column: CYCLOBOND I 2000 AC, 25 cm x 4.6 mm I.D., 5 μm particles (20124AST)
mobile phase: [A] 1.0% triethylamine acetate, pH 4.1; [B] acetonitrile; (85:15, A:B)
flow rate: 1 mL/min
column temp.: 22 °C
detector: UV, 254 nm
injection: 2 μL
sample: 1 mg/mL in methanol
peak 1: 9.48 min.
peak 2: 12.48 min.

适用性

application for HPLC

应用

pharmaceutical (small molecule)