HPLC Analysis of Warfarin Enantiomers on Astec^® CHIROBIOTIC^® V2

技术

HPLC: suitable

试验参数

column: CHIROBIOTIC V2, 25 cm x 4.6 mm I.D., 5 μm particles (15024AST)
mobile phase: [A] 10 mM ammonium acetate, pH 4.1; [B] acetonitrile; (70:30, A:B)
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 280 nm
injection: 10 μL
sample: 1 mg/mL in methanol

适用性

application for HPLC

应用

clinical
pharmaceutical (small molecule)