HPLC Analysis of Verapamil and Metabolites in Rat Plasma on Ascentis^® Express C18 after Phospholipid Removal using HybridSPE^®-Phospholipid

技术

LC/MS: suitable
solid phase extraction (SPE): suitable

试验参数

SPE well plate: HybridSPE-Precipitation 96-well Plate, 50 mg/well (575656-U)
sample preparation: SPE (Solid Phase Extraction)
column: Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)
sample/matrix: Rat plasma spiked with verapamil and metabolites at 10 ng/mL
mobile phase: [A] water; [B] acetonitrile, pH 2.55 adjust with formic acid (30:70, A:B)
flow rate: 0.6 mL/min
column temp.: 35 °C
detector: TOF/MS
injection: 1 μL
sample addition: 100 μL spiked rat plasma followed by 300 μL 1% formic acid in acetonitrile
elution: apply vacuum

适用性

application for LC-MS
application for SPE

应用

clinical