HPLC Analysis of Hydrocortisone in Topical Cream on Discovery^® HS C18 after SPE using Discovery^® DSC-Si

技术

HPLC: suitable
solid phase extraction (SPE): suitable

试验参数

sample preparation: SPE (Solid Phase Extraction)
sample addition: hydrocortisone topical hand cream in hexane:ethylacetate
SPE tube/cartridge: Discovery DSC-Si, 500 mg/3 mL (52695-U)
condition: 1 mL hexane:ethyl acetate (2:1, v/v)
sample addition: 1 mL
washing: 1 mL hexane:ethyl acetate (2:1, v/v)
elution: 1 mL methanol:DI water (1:1, v/v)
eluate post-treatment: dilute eluate to 2 mL with methanol:DI water (1:1, v/v)
column: Discovery HS C18, 15 cm x 4.6 mm I.D., 5 μm particles (568520-U)
mobile phase: [A] methanol; [B] DI water (1:1, A:B)
flow rate: 1 mL/min
column temp.: 35 °C
detector: UV, 220 nm
injection: 10 μL

适用性

application for HPLC
application for SPE

应用

cleaning products
cosmetics
personal care
pharmaceutical (small molecule)