HPLC Analysis of Cortocisteroids in Urine on Discovery^® HS F5 after SPE using Discovery^® DSC-CN

技术

HPLC: suitable
solid phase extraction (SPE): suitable

试验参数

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: human urine spiked with corticosteroids at 0.5 and 1.0 μg/mL and diluted in DI water (1:1, v/v)
SPE well plate: Discovery DSC-CN 96-well SPE, 100 mg/well (575636-U)
condition: 1 mL methanol; 1 mL DI water.
sample addition: 1 mL spiked urine
washing: 1 mL 20% methanol
elution: 1 mL methanol
eluate post-treatment: Evaporate eluate with nitrogen at 30 °C. Reconstitute in 200 μL mobile phase.
column: Discovery HS F5, 5 cm x 4.6 mm I.D., 3 μm particles (567504-U)
mobile phase: [A] methanol; [B] DI water; (40:60, A:B)
flow rate: 1.5 mL/min
column temp.: 35 °C
detector: UV, 240 nm
injection: 5 μL

适用性

application for HPLC
application for SPE

应用

clinical