LC/MS Analysis of Cannabinoids on Ascentis^® RP-Amide

技术

HPLC: suitable

试验参数

column: Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)
mobile phase: [A] 10 mM ammonium acetate (pH 5.0 with acetic acid): [B] acetonitrile; (20:80, A:B)
flow rate: 1 mL/min, split to the MS
column temp.: 35 °C
detector: MS ESI (+), SIR Mode
injection: 5 μL
sample: 1 μg/mL in water:acetonitrile (1:1)

适用性

application for HPLC

应用

forensics and toxicology
pharmaceutical (small molecule)