HPLC Analysis of Antihypertensive Drugs on Ascentis^® RP-Amide (UV, 254 nm)

技术

HPLC: suitable

试验参数

column: Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)
mobile phase: [A] 10 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid); [B] acetonitrile; (80:20, A:B)
flow rate: 1 mL/min
column temp.: 35 °C
detector: UV, 254 nm
injection: 10 μL
sample: 50 μg/mL each in mobile phase

适用性

application for HPLC

应用

pharmaceutical (small molecule)