HPLC Analysis of Serotonin Metabolites on Ascentis^® C18

技术

HPLC: suitable

试验参数

column: Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)
mobile phase: [A] 10 mM formic acid/acetate (pH 3.0): [B] methanol; (80:20, A:B)
flow rate: 0.7 mL/min
column temp.: 35 °C
detector: ESI (+); overlay of extracted ion chromatograms of individual (M+H)+ species
injection: 10 μL
sample: 25 mg/L in 90:10, 10 mM formic acid/acetate (pH 3.0):methanol

适用性

application for HPLC

应用

clinical