HPLC Analysis of Warfarin Anticoagulant on Ascentis^® C18

技术

HPLC: suitable

试验参数

column: Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)
mobile phase: [A] 20 mM phosphoric acid (pH 2.0, unadjusted): [B] methanol; (30:70, A:B)
flow rate: 1 mL/min
column temp.: 35 °C
detector: UV, 280 nm
injection: 10 μL
sample: 50 μg/mL each in mobile phase

适用性

application for HPLC

应用

clinical
pharmaceutical (small molecule)