HPLC Analysis of Peptides on Discovery^® BIO Wide Pore C5, 0-16 Min. Window

技术

HPLC: suitable

试验参数

column: Discovery BIO Wide Pore C5, 15 cm × 4.6 mm I.D., 5 μm particles (568422-U)
mobile phase: [A] (95:5), 0.1% TFA in water:0.1% TFA acetonitrile; [B] (50:50), 0.1% TFA in water:0.1% TFA in acetonitrile
gradient: 0-100% B in 65 min
flow rate: 1 mL/min
column temp.: 30 °C
detector: UV, 215 nm
injection: 50μL carboxymethylated apohemoglobin tryptic digest in 50 mM ammonium bicarbonate (568422-U)

适用性

application for HPLC

应用

life science and biopharma