技术
HPLC: suitable
试验参数
column: Discovery BIO Wide Pore C5, 15 cm × 4.6 mm I.D., 5 μm particles (568422-U)
mobile phase: [A]: 71:29, (0.1% TFA in water):(0.1% TFA in acetonitrile);
[B]: 68:32, (0.1% TFA in water):(0.1% TFA in acetonitrile)
gradient: 0-100% B in 30 min
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 215 nm
injection: 5 μL 5 μL in 0.1% TFA containing 5ug each of three insulin variants
适用性
application for HPLC
应用
life science and biopharma
pharmaceutical (small molecule)
法律信息
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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