技术
HPLC: suitable
试验参数
column: Discovery BIO Wide Pore C5, 15 cm × 4.6 mm I.D., 5 μm particles (568422-U)
mobile phase: [A] 0.1% TFA in water:0.1% TFA in acetonitrile, 95:5; [B] 0.1% TFA in water:0.1% TFA in acetonitrile; 50:50
gradient: 0-100% B in 65 min
flow rate: 1 mL/min
column temp.: 30 °C
detector: UV, 215 nm
injection: 50 μL
sample: carboxymethylated apohemoglobin tryptic digest in 50 mM ammonium bicarbonate
适用性
application for HPLC
应用
life science and biopharma
法律信息
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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