HPLC Analysis of Tricyclic Antidepressants in Serum on Discovery^® C18 after SPE using Discovery^® DSC-18

技术

HPLC: suitable
solid phase extraction (SPE): suitable

试验参数

sample preparation: SPE (Solid Phase Extraction)
SPE tube/cartridge: Discovery DSC-18, 100 mg/1 mL (52602-U)
column: Discovery C18, 15 cm × 4.6 mm, 5 μm preceded by a 2 cm C18 guard column and 0.5 μm frit filter (504955)
mobile phase: [A] acetonitrile: [B] methanol: [C] 25 mM potassium phosphate, monobasic (pH 7 with triethlylamine) (45:25:30, A:B:C)
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 254 nm
injection: 50 μL, diluted porcine serum extract

适用性

application for HPLC
application for SPE

应用

clinical