HPLC Analysis of Nonvolatile Pesticides in Water on SUPELCOSIL^™ LC-18-DB after SPE using Supelclean^™ ENVI-Carb^™

技术

HPLC: suitable
solid phase extraction (SPE): suitable

试验参数

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: water spiked with pesticides at 10-50 μg/L
SPE tube/cartridge: Supelclean ENVI-Carb, 250 mg/3 mL (57088)
condition: 5 mL methylene chloride:methanol (80:20); 1 mL methanol; 10 mL 2% acetic acid in water (keep bed moist until sample addition)
sample addition: 100 mL sample at 5 mL/min
drying: 1 minute with vacuum
elution: 0.8 mL methanol; 2 x 35 mL methylene chloride:methanol (80:20)
eluate post-treatment: dry eluate to 500 μL under gentle nitrogen stream, reconstitute to 1 mL with methanol
column: SUPELCOSIL LC-18-DB, 25 cm × 4.6 mm I.D., 5 μm particles (58355-U)
mobile phase: [A] water:acetonitrile (90:10), [B] acetonitrile; gradient 80% A for 5 min then to 30% A over 30 min
flow rate: 1.5 mL/min
detector: UV, 220 nm
injection: 20 μL

适用性

application for HPLC
application for SPE

应用

environmental