TOYOPEARL^® 疏水相互作用介质

TOYOPEARL^® HIC 树脂用于疏水相互作用层析，具有以下优点:

• 对水溶性蛋白亲和力强
• 质量和活性回收率高
• 样品容量大:高达凝胶介质的 2-4 倍
• 盐浓度的波动不会改变柱床体积
• 机械稳定性达 7kg/cm² (7 bar/100psi)
• 宽 pH 值稳定范围:2-12
• 用 0.5M NaOH 在线清洗
• 可高压灭菌

     
TOYOPEARL 介质使用各种水性洗脱剂可得高蛋白回收率。5x10⁶Da 的排阻限和 1000Å 的大孔径使得这些填料能够通过疏水相互作用机理分离非常大的蛋白质，而不会产生分子筛效应。100mL/瓶。

TOYOPEARL^® HIC resins are hydrophobic interaction chromatography resins that offer the following advantages: strong affinity for water-soluble proteins, high recovery of mass and activity, high sample capacity up to 2-4 times that of gel media, fluctuating salt concentrations will not change the bed volume, mechanical stability to 7kg/cm squared (7 bar/100psi), stability at a wide pH range (2-12), clean in place with 0.5M NaOH, and autoclavable. The exclusion limit of 5x10⁶ Da and large pore size, 1000Å, enables these packings to separate very large proteins by a hydrophobic interaction mechanism, without size exclusion effects.

Clean in place with 0.5 M NaOH or 0.1 M HCl.

72% (v/v) slurry in 20% (v/v) ethanol

Toyopearl Butyl-650 resin has the second highest hydrophobicity of the HIC ligands offered by TBL. Its pore size is the largest of our three butyl resins. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in interresinte purification and polishing steps

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Toyopearl is a registered trademark of Tosoh Corporation