产品名称
TSK-GEL® 体积排阻（SW 类型）高效液相色谱柱, phase diol, L × I.D. 30 m × 7.8 mm, 4 μm particle size
material
stainless steel
agency
suitable for USP L20
suitable for USP L59
product line
TSKgel®
manufacturer/tradename
Tosoh SuperSW mAb HR, 22854
parameter
≤0.5 M salt concentration
0-100% aqueous soluble organic solvents
1 mL/min flow rate
10-30 °C temperature
120 bar max. pressure
technique(s)
HPLC: suitable
L × I.D.
30 m × 7.8 mm
matrix
silica particle platform
matrix active group
diol phase
particle size
4 μm
pore size
250 Å pore size
operating pH
2.5-7.5
separation technique
size exclusion (SEC)
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Physical form
Shipped in 0.05% NaN3 and 0.1 M Na2SO4 in 0.1 M phosphate buffer, pH 6.7.
Legal Information
TSKgel is a registered trademark of Tosoh Corporation
Application
TSKgel® superSW mAb HR HPLC column may be used in the separation of monoclonal antibodies using size exclusion chromatography (SEC).
General description
The TSKgel SuperSW and UltraSW mAb columns consist of three specialized columns designed for the separation and analysis of monoclonal antibodies (mAb). While mAbs can be analyzed using many different modes of HPLC, size exclusion is best to determine the level of aggregation, dimer formation, and fragmentation, making it the best method for heterogeneity studies. TSKgel SuperSW mAb HR columns are optimized for high resolution analysis of mAb monomer and dimer; these 30 cm x 7.8 mm ID slica-based stainless steel columns offer reduced lot-to-lot variation, long column life, reduction of unspecified adsorption, and superior recovery of aggregates. The TSKgel SuperSW mAb columns utilize a unique pore-controlled technology, which produces a shallow calibration curve in the molecular weight region of a typical monoclonal antibody. The calibration curve for the TSKgel SuperSW mAb HR column is similar to that of the industry-leading TSKgel G3000SWxl column and has a shallower slope than the TSKgel UltraSW Aggregate column around the molecular weight range of gamma-globulin. This shallow calibration curve produces high resolution separations.
法规信息
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Alexander Rau et al.
mAbs, 13(1), 1902034-1902034 (2021-03-24)
Dual targeting of surface receptors with bispecific antibodies is attracting increasing interest in cancer therapy. Here, we present a novel bivalent and bispecific antagonistic molecule (Dab-Fc) targeting human epidermal growth factors 2 and 3 (HER2 and HER3) derived from the
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Alexander Rau et al.
Molecular cancer therapeutics, 19(7), 1474-1485 (2020-05-21)
The frequent activation of HER3 signaling as a resistance mechanism to EGFR-targeted therapy has motivated the development of combination therapies that block more than one receptor tyrosine kinase. Here, we have developed a novel tetravalent, bispecific single-chain diabody-Fc fusion protein
商品
Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.
体积排阻色谱（SEC）色谱柱和现成的标准品有助于方法开发，并提高蛋白质SEC方法的耐用性。
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