product name
TSKgel® Phenyl-5PW RP HPLC Column, phase phenyl, L × I.D. 15 cm × 21.5 mm, 13 μm particle size
物料
stainless steel hardware
产品线
TSKgel®
制造商/商品名称
Tosoh Phenyl-5PW, 16260
标记范围
1 meq/mL ligand density (estimate)
参数
no limitation salt concentration
0-100% organic solvent
30 bar max. pressure
4-60 °C temperature
8 mL/min flow rate
技术
HPLC: suitable
长度 × 内径
15 cm × 21.5 mm
基质
polymer particle platform
基质活性基团
phenyl phase
粒径
13 μm
孔径
1000 Å
工作pH值
2-12
分离技术
reversed phase
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一般描述
TSKgel Phenyl-5PW RP offers similiar selectivity to its hydrophobic interation counterpart TSKgel Phenyl-5PW, although a higher degree of cross-linking allows for increased stability in aqueous/organic mobile phase gradients. The highly cross-linked polymethacrylate base material provides an advantage over silica when high pH buffer systems are needed. Additionally, TSKgel Phenyl-5PW RP can withstand rigorous cleaning protocols using either acid or base. The 1000Å pore size accommodates globular protein samples up to 1,000,000 Da.
Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
- Octadecyl-2PW particles have 125Å pores
- Octadecyl-4PW particles have 500Å pores
- Phenyl-5PW RP particles have 1000Å pores
- Octadecyl-NPR particles are nonporous
外形
Shipped in 30% methanol - 70% water.
法律信息
TSKgel is a registered trademark of Tosoh Corporation
法规信息
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