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描述
AF-Chelate-650M
产品线
TOYOPEARL®
表单
liquid
包装
bottle of 25 mL
制造商/商品名称
Tosoh TOYOPEARL® AF, 14475
标记范围
25-45 μeq/mL ligand density
参数
3 bar max. pressure
技术
HPLC: suitable
基质
hydroxylated methacrylic polymer
基质活性基团
IDA phase, iminodiacetic acid
粒径
65 μm
工作pH值
2-12
分离技术
affinity
储存温度
2-8°C
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一般描述
Toyopearl AF-Chelate-650 resin is derivatized with iminodiacetic acid for immobilized metal affinity chromatography or IMAC.IMAC works primarily by binding to histidine and free cysteine containing sequences of proteins or peptides.
应用
TOYOPEARL® Affinity Media are used in affinity chromatography, protein chromatography and activated/functionalized matrices.
产品规格
Clean in place with 0.5 M NaOH or 0.1 M HCl.
外形
Shipped in 20% (v/v) ethanol.
法律信息
Toyopearl is a registered trademark of Tosoh Corporation
警示用语：
Warning
危险声明
危险分类
Flam. Liq. 3
WGK
WGK 1
闪点(°F)
103.1 °F
闪点(°C)
39.5 °C
法规信息
新产品
S Knör et al.
Journal of thrombosis and haemostasis : JTH, 6(3), 470-477 (2008-01-16)
Hemophilia A is currently treated by infusions of the coagulation factor (F) VIII, of which production and purification remain a challenging task. Current purification procedures using immunoaffinity chromatography are cumbersome, expensive, and suffer from the instability of the applied antibody
S Yamauchi et al.
The Journal of biological chemistry, 274(4), 2456-2463 (1999-01-16)
Chondroitin 4-sulfotransferase, which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 4 of N-acetylgalactosamine in chondroitin, was purified 1900-fold to apparent homogeneity with 6.1% yield from the serum-free culture medium of rat chondrosarcoma cells by affinity chromatography on heparin-Sepharose CL-6B, Matrex
Zhenzhen Wen et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(20), 1732-1740 (2011-05-17)
In order to improve its stability, immobilized Concanavalin A (Con A) on Toyopearl adsorbents was conjugated with monomethoxy poly(ethylene glycol) succinimidyl propionate (mPEG-SPA) with different molecular weight. A colorimetric method using ninhydrin is proposed to determine the degree of PEGylation;
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