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产品名称
TSKgel® Boronate-5PW HPLC Column, phase m-aminophenyl boronate, L × I.D. 5 cm × 5 mm, 10 μm particle size, glass
物料
glass
产品线
TSKgel®
分析物化学类别
proteins
制造商/商品名称
Tosoh 14449
参数
≤20% organic solvent
≤3 M salt concentration
>0 °C temperature
1.2 mL/min flow rate
30 bar max. pressure
技术
HPLC: suitable
长度 × 内径
5 cm × 5 mm
基质
hydroxylated methacrylic polymer
基质活性基团
m-aminophenyl boronate phase
粒径
10 μm
工作pH值
2-9
容量
40 μmol/mL, resin adsorption capacity (sorbitol)
分离技术
affinity
储存温度
2-8°C
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一般描述
High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.
Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.
Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.
Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.
Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.
法律信息
TSKgel is a registered trademark of Tosoh Corporation
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