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产品名称
TSKgel® Ether-5PW HPLC Column, phase ether, L × I.D. 7.5 cm × 8 mm, 10 μm particle size
物料
glass
产品线
TSKgel®
制造商/商品名称
Tosoh 14014
参数
≤4 M salt concentration
≤50% organic solvent
1.2 mL/min flow rate
10-50 °C temperature
20 bar max. pressure
技术
HPLC: suitable
长度 × 内径
7.5 cm × 8 mm
基质
hydroxylated methacrylic polymer
基质活性基团
ether phase
粒径
10 μm
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此商品 | EPM11909000 | EPACSB1000S | EPACE2000S |
---|---|---|---|
manufacturer/tradename Eppendorf® ACE-10S (Global) | manufacturer/tradename Eppendorf® M1190-9000 (Global) | manufacturer/tradename Eppendorf® ACSB-1000S (Global) | manufacturer/tradename Eppendorf® ACE-2000S (Global) |
W × H 31 mm × 26 mm | W × H 51 mm × 26 mm | W × H 129 mm × 146 mm | W × H 152 mm × 71 mm |
一般描述
Three resin-based TSKgel HIC columns provide a range of hydrophobicities for chromatographic optimization. TSKgel Phenyl-5PW columns are most hydrophobic, followed by Ether-5PW, while the surface of Butyl-NPR columns is most polar or least hydrophobic. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing such as Phenyl-5PW. We recommend the more polar Ether-5PW column for purifying more hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000  pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.
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