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产品名称
TSKgel® Ether-5PW HPLC Column, phase ether, L × I.D. 7.5 cm × 8 mm, 10 μm particle size
物料
glass
产品线
TSKgel®
制造商/商品名称
Tosoh 14014
参数
≤4 M salt concentration
≤50% organic solvent
1.2 mL/min flow rate
10-50 °C temperature
20 bar max. pressure
技术
HPLC: suitable
长度 × 内径
7.5 cm × 8 mm
基质
hydroxylated methacrylic polymer
基质活性基团
ether phase
粒径
10 μm
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此商品 | 814013 | 808641 | 818760 |
|---|---|---|---|
| matrix active group ether phase | matrix active group ether phase | matrix active group ether phase | matrix active group ether phase |
| separation technique hydrophobic interaction (HIC) | separation technique hydrophobic interaction (HIC) | separation technique hydrophobic interaction (HIC) | separation technique hydrophobic interaction (HIC) |
| particle size 10 μm | particle size 10 μm | particle size 10 μm | particle size 10 μm |
| technique(s) HPLC: suitable | technique(s) HPLC: suitable | technique(s) HPLC: suitable | technique(s) HPLC: suitable |
| product line TSKgel® | product line TSKgel® | product line TSKgel® | product line TSKgel® |
一般描述
Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.
Three resin-based TSKgel HIC columns provide a range of hydrophobicities for chromatographic optimization. TSKgel Phenyl-5PW columns are most hydrophobic, followed by Ether-5PW, while the surface of Butyl-NPR columns is most polar or least hydrophobic. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing such as Phenyl-5PW. We recommend the more polar Ether-5PW column for purifying more hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000  pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.
Three resin-based TSKgel HIC columns provide a range of hydrophobicities for chromatographic optimization. TSKgel Phenyl-5PW columns are most hydrophobic, followed by Ether-5PW, while the surface of Butyl-NPR columns is most polar or least hydrophobic. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing such as Phenyl-5PW. We recommend the more polar Ether-5PW column for purifying more hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000  pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.
法律信息
TSKgel is a registered trademark of Tosoh Corporation
法规信息
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