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814014

TSKgel® Ether-5PW (10 µm) HPLC Columns

L x I.D. 7.5 cm × 8 mm, ether phase

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CN¥18,838.04

CN¥18,838.04


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1 EA
CN¥18,838.04

About This Item

UNSPSC代码:
41115711

CN¥18,838.04


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产品名称

TSKgel® Ether-5PW HPLC Column, phase ether, L × I.D. 7.5 cm × 8 mm, 10 μm particle size

物料

glass

产品线

TSKgel®

制造商/商品名称

Tosoh 14014

参数

≤4 M salt concentration
≤50% organic solvent
1.2 mL/min flow rate
10-50 °C temperature
20 bar max. pressure

技术

HPLC: suitable

长度 × 内径

7.5 cm × 8 mm

基质

hydroxylated methacrylic polymer

基质活性基团

ether phase

粒径

10 μm

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814013808641818760
matrix active group

ether phase

matrix active group

ether phase

matrix active group

ether phase

matrix active group

ether phase

separation technique

hydrophobic interaction (HIC)

separation technique

hydrophobic interaction (HIC)

separation technique

hydrophobic interaction (HIC)

separation technique

hydrophobic interaction (HIC)

particle size

10 μm

particle size

10 μm

particle size

10 μm

particle size

10 μm

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

product line

TSKgel®

product line

TSKgel®

product line

TSKgel®

product line

TSKgel®

一般描述

Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.
Three resin-based TSKgel HIC columns provide a range of hydrophobicities for chromatographic optimization. TSKgel Phenyl-5PW columns are most hydrophobic, followed by Ether-5PW, while the surface of Butyl-NPR columns is most polar or least hydrophobic. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing such as Phenyl-5PW. We recommend the more polar Ether-5PW column for purifying more hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000  pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.

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法律信息

TSKgel is a registered trademark of Tosoh Corporation

法规信息

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