产品名称
TSKgel® Octadecyl-NPR HPLC Column, phase C18 (octadecyl), L × I.D. 3.5 cm × 4.6 mm, 2.5 μm particle size
material
stainless steel
product line
TSKgel®
manufacturer/tradename
Tosoh 14005
extent of labeling
1 meq/mL ligand density (estimate)
parameter
≤0.5 M salt concentration, 0-100% organic solvent, 0-60 °C temperature, 1.6 mL/min flow rate, 200 bar max. pressure
technique(s)
HPLC: suitable
L × I.D.
3.5 cm × 4.6 mm
matrix
polymer particle platform
matrix active group
C18 (octadecyl) phase
particle size
2.5 μm
operating pH
2-12
separation technique
reversed phase
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General description
Highly cross-linked polymethacrylate base material of TSKgel Octadecyl-NPR provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. NPR, non-porous resin, columns are prepared from non-porous methacrylate particles of uniform 2.5 micron size, which provides high efficiency separations and fast anaylses of peptides and proteins. The non-porous particle structure limits product isolation to sub-microgram loads.
Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
- Octadecyl-2PW particles have 125Å pores
- Octadecyl-4PW particles have 500Å pores
- Phenyl-5PW RP particles have 1000Å pores
- Octadecyl-NPR particles are nonporous
Physical form
Shipped in 70% methanol - 30% waters (v/v).
Legal Information
TSKgel is a registered trademark of Tosoh Corporation
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