推荐产品
产品名称
TSKgel® Boronate-5PW HPLC Column, phase m-aminophenyl boronate, L × I.D. 7.5 cm × 7.5 mm, 10 μm particle size
物料
PEEK
产品线
TSKgel®
分析物化学类别
proteins
制造商/商品名称
Tosoh Boronate-5PW, 13066
参数
≤20% organic solvent
≤3 M salt concentration
1.2 mL/min flow rate
30 bar max. pressure
≥ 0 °C temperature
技术
HPLC: suitable
长度 × 内径
7.5 cm × 7.5 mm
基质
polymer particle platform
基质活性基团
m-aminophenyl boronate phase
粒径
10 μm
孔径
1000 Å
工作pH值
2-9
容量
40 μmol/mL adsorption capacity (sorbitol)
相容性
mode of use affinity HPLC
分离技术
affinity
储存温度
2-8°C
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一般描述
High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.
Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.
Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.
Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.
Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.
法律信息
TSKgel is a registered trademark of Tosoh Corporation
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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