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描述
AF-Amino-650M
产品线
TOYOPEARL®
表单
liquid
包装
bottle of 25 mL
制造商/商品名称
Tosoh TOYOPEARL® AF, 08002
标记范围
100 μmol/mL±30 ligand density
参数
3 bar max. pressure
技术
HPLC: suitable
基质
hydroxylated methacrylic polymer
基质活性基团
amine-activated phase
粒径
65 μm
工作pH值
2-13
分离技术
anion exchange
储存温度
2-8°C
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一般描述
Toyopearl AF-Amino-650 resin is a reactive resin used for the coupling of specific ligands for affinity chromatography. Ligands are immobilized by either peptide bond formation or reductive amination through their respective carboxylate or aldehyde groups.
应用
TOYOPEARL® Affinity Media are used in affinity chromatography, protein chromatography and activated/functionalized matrices.
外形
Shipped in 20% (v/v) ethanol.
法律信息
Toyopearl is a registered trademark of Tosoh Corporation
警示用语：
Warning
危险声明
危险分类
Flam. Liq. 3
WGK
WGK 1
个人防护装备
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
新产品
S Knör et al.
Journal of thrombosis and haemostasis : JTH, 6(3), 470-477 (2008-01-16)
Hemophilia A is currently treated by infusions of the coagulation factor (F) VIII, of which production and purification remain a challenging task. Current purification procedures using immunoaffinity chromatography are cumbersome, expensive, and suffer from the instability of the applied antibody
S Yamauchi et al.
The Journal of biological chemistry, 274(4), 2456-2463 (1999-01-16)
Chondroitin 4-sulfotransferase, which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 4 of N-acetylgalactosamine in chondroitin, was purified 1900-fold to apparent homogeneity with 6.1% yield from the serum-free culture medium of rat chondrosarcoma cells by affinity chromatography on heparin-Sepharose CL-6B, Matrex
Zhenzhen Wen et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(20), 1732-1740 (2011-05-17)
In order to improve its stability, immobilized Concanavalin A (Con A) on Toyopearl adsorbents was conjugated with monomethoxy poly(ethylene glycol) succinimidyl propionate (mPEG-SPA) with different molecular weight. A colorimetric method using ninhydrin is proposed to determine the degree of PEGylation;
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