产品线
TOYOPEARL®
形式
powder
包装
bottle of 10 g
制造商/商品名称
Tosoh TOYOPEARL® AF, 08000
标记范围
800 μeq/g ligand density
参数
3 bar max. pressure
技术
HPLC: suitable
基质
hydroxylated methacrylic polymer
基质活性基团
expoxy-activated phase
粒径
65 μm
容量
>60 g/L adsorption capacity (soybean trypsin inhibitor)
分离技术
affinity
储存温度
2-8°C
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一般描述
Toyopearl AF-Epoxy-650 resin is an activated resin provided in dry form for the immobilization of protein ligands for affinity chromatography. It is used when high densities of low molecular weight molecules need to be attached. It is also useful when a conversion to other special functional groups is required prior to ligand immobilization. For instance, its hydrazide form is very useful for carbohydrates or glycoprotein ligands.
应用
TOYOPEARL® Affinity Media are used in affinity chromatography, protein chromatography and activated/functionalized matrices.
外形
Shipped as dry, free flowing powder.
法律信息
Toyopearl is a registered trademark of Tosoh Corporation
警示用语：
Warning
危险声明
危险分类
Flam. Liq. 3
WGK
WGK 3
闪点(°F)
closed cup
闪点(°C)
closed cup
法规信息
新产品
S Yamauchi et al.
The Journal of biological chemistry, 274(4), 2456-2463 (1999-01-16)
Chondroitin 4-sulfotransferase, which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 4 of N-acetylgalactosamine in chondroitin, was purified 1900-fold to apparent homogeneity with 6.1% yield from the serum-free culture medium of rat chondrosarcoma cells by affinity chromatography on heparin-Sepharose CL-6B, Matrex
S Knör et al.
Journal of thrombosis and haemostasis : JTH, 6(3), 470-477 (2008-01-16)
Hemophilia A is currently treated by infusions of the coagulation factor (F) VIII, of which production and purification remain a challenging task. Current purification procedures using immunoaffinity chromatography are cumbersome, expensive, and suffer from the instability of the applied antibody
Zhenzhen Wen et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(20), 1732-1740 (2011-05-17)
In order to improve its stability, immobilized Concanavalin A (Con A) on Toyopearl adsorbents was conjugated with monomethoxy poly(ethylene glycol) succinimidyl propionate (mPEG-SPA) with different molecular weight. A colorimetric method using ninhydrin is proposed to determine the degree of PEGylation;
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