TSKgel^® Phenyl-5PW (10 µm) HPLC Columns

The most hydrophobic among the three TSKgel HIC columns, TSKgel Phenyl-5PW is an excellent choice to screen for the selectivity, retention and recovery of most biomolecules. Featuring a 1000Å pore size in a polymethacrylate base material, the Phenyl-5PW column is stable in either acid or caustic cleaning regimens and provides excellent access to larger molecules with low diffusion coefficients. Stainless steel is the standard column format for TSKgel Phenyl-5PW. Glass and PEEK column housings are available for samples that may adsorb to stainless steel. The alternative column formats are also suitable for general applications.

疏水作用色谱法(HIC)和反相液相色谱法均基于蛋白质疏水性进行分离，并允许蛋白质的选择性结合和解吸。但是，HIC在明显较低的结合能下运行，并使用水性流动相。这些特征提供了一种温和的技术，不太可能干扰蛋白质构象。结果，HIC通常提供更好的活性恢复。

TSKgel BioAssist苯基色谱柱含有与Phenyl-5PW色谱柱相同的颗粒，但填充在PEEK色谱柱硬件中，而不是不锈钢。BioAsist苯基（Phenyl-5PW）、TSKgel Ether-5PW和Butyl-NPR树脂色谱柱为色谱优化提供了一系列疏水性。可以通过使用更疏水的填料（例如BioAssist Phenyl）将硫酸铵的浓度降至最低。我们建议使用Ether-5PW色谱柱纯化非常疏水的蛋白质。TSKgel BioAssist苯基和TSKgel Ether-5PW填料基于TSKgel G5000PW树脂 - 10 μm颗粒，具有1000 Å孔。疏水性低于Ether-5PW的TSKgel Butyl-NPR色谱柱由2.5 μm无孔颗粒制备，允许快速分析和半微制备应用。所有三种色谱柱类型均可用0.2 M NaOH清洗。 可通过使用相应的Toyopearl散装树脂进行放大。

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