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TOYOPEARL® 疏水相互作用介质

matrix polymer (65 μm), bottle of 100 mL, 65 μm

别名:

TOYOPEARL® Butyl-650M

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About This Item

UNSPSC代码:
41115700

描述

Butyl-650M

产品线

TOYOPEARL®

表单

slurry

包装

bottle of 100 mL

参数

3 bar max. pressure

技术

HPLC: suitable

基质

polymer (65 μm)

基质活性基团

C4 phase

粒径

65 μm

工作pH值

1-13

容量

40 g/L±10 adsorption capacity (lysozyme)

分离技术

hydrophobic interaction (HIC)

一般描述

TOYOPEARL® HIC 树脂用于疏水相互作用层析，具有以下优点:
  • 对水溶性蛋白亲和力强
  • 质量和活性回收率高
  • 样品容量大:高达凝胶介质的 2-4 倍
  • 盐浓度的波动不会改变柱床体积
  • 机械稳定性达 7kg/cm2 (7 bar/100psi)
  • 宽 pH 值稳定范围:2-12
  • 用 0.5M NaOH 在线清洗
  • 可高压灭菌
     
TOYOPEARL 介质使用各种水性洗脱剂可得高蛋白回收率。5x106Da 的排阻限和 1000Å 的大孔径使得这些填料能够通过疏水相互作用机理分离非常大的蛋白质，而不会产生分子筛效应。100mL/瓶。
TOYOPEARL® HIC resins are hydrophobic interaction chromatography resins that offer the following advantages: strong affinity for water-soluble proteins, high recovery of mass and activity, high sample capacity up to 2-4 times that of gel media, fluctuating salt concentrations will not change the bed volume, mechanical stability to 7kg/cm squared (7 bar/100psi), stability at a wide pH range (2-12), clean in place with 0.5M NaOH, and autoclavable. The exclusion limit of 5x106 Da and large pore size, 1000Å, enables these packings to separate very large proteins by a hydrophobic interaction mechanism, without size exclusion effects.

应用

TOYOPEARL® media are used in hydrophobic interaction media, resins and separation media. TOYOPEARL® media offer high yield recovery of proteins, using various aqueous eluants.

产品规格

Clean in place with 0.5 M NaOH or 0.1 M HCl.

外形

Shipped in 20% (v/v) ethanol.

其他说明

Toyopearl Butyl-650 resin has the second highest hydrophobicity of the HIC ligands offered by TBL. Its pore size is the largest of our three butyl resins. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in interresinte purification and polishing steps

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法律信息

Toyopearl is a registered trademark of Tosoh Corporation

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警示用语：

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危险声明

H226

危险分类

Flam. Liq. 3

WGK

WGK 1

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

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Hongwei Luan et al.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 24(5), 867-873 (2008-08-30)
A beta-D-xylosidase from Leifsonia shinshuensis DICP 16 was purified to apparent homogeneity using a combination of ammonium sulfate precipitation, DE 52 anion-exchange, Q-Sepharose Fast Flow anion-exchange, Toyopearl Butyl 650C hydrophobic-interaction and Sephacryl S-300 HR gel-permeation chromatography. The purified xylosidase consisted
Hydrophobic interaction chromatography selectivity changes among three stable proteins: conformation does not play a major role
Jones, T.T. and Fernandez, E.J.
Biotechnology and Bioengineering, 87, 288-299 (2004)
P Ceccaroli et al.
Journal of chromatography. B, Biomedical sciences and applications, 702(1-2), 41-48 (1998-02-04)
Hydrophobic interaction chromatography (HIC) has been used extensively for the separation of proteins and peptides by elution using a descending salt gradient, with and without the use of detergents or denaturing agents. In this paper we compare different hydrophobic interaction
V Stocchi et al.
Journal of chromatography. A, 676(1), 51-63 (1994-07-29)
Hydrophobic interaction chromatography (HIC) has been employed extensively in the separation of proteins by elution using a descending salt gradient, with and without the use of detergents or denaturing agents. In this study, a new hydrophobic interaction chromatographic support, Toyopearl

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