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描述
DEAE-650M
形式
slurry
特点
counter ion Cl-
包装
bottle of 250 mL
参数
3 bar max. pressure
技术
HPLC: suitable
基质
methacrylate
基质活性基团
DEAE (diethylaminoethyl) phase
粒径
65 μm
工作pH值
2-10
容量
0.10 meq/mL±0.02 meq/mL ion exchange capacity
30 g/L±5 adsorption capacity (BSA)
30 mg/mL±5 mg/mL binding capacity (bovine serum albumin)
相容性
mode of use weak anion exchange chromatography
分离技术
anion exchange
一般描述
Toyopearl DEAE-650 resin is a weak anion exchanger for blood fractionation and other biomolecule purification. It can be used for high throughput capture, intermediate purification, and polishing process steps. If more powerful resolution is needed TSKgel DEAE-5PW resin should be used. Selectivity remains the same.
应用
TOYOPEARL® DEAE-650M is used in separation media and ion exchange resins. TOYOPEARL® DEAE-650M has been used in haemophilia studies.
产品规格
Clean in place with 0.5 M NaOH or 0.1 M HCl.
外形
Shipped in 20% (v/v) ethanol.
法律信息
Toyopearl is a registered trademark of Tosoh Corporation
警示用语：
Warning
危险声明
危险分类
Flam. Liq. 3
WGK
WGK 1
个人防护装备
Eyeshields, Gloves, type N95 (US)
P R Foster et al.
Vox sanguinis, 86(2), 92-99 (2004-03-17)
The risk of haemophiliacs contracting variant Creutzfeldt-Jakob disease (vCJD) via treatment with factor VIII concentrates is not known. Therefore, in order to determine the extent to which the vCJD agent might be removed during the preparation of factor VIII concentrate
Christine Machold et al.
Journal of biotechnology, 117(1), 83-97 (2005-04-16)
Two different approaches of matrix assisted refolding have been evaluated and compared to conventional refolding by dilution. Bovine alpha-lactalbumin was used for the studies as model protein. It was adsorbed under denaturing conditions on an ion exchange matrix and refolding
Günter Iberer et al.
Journal of chromatography. A, 972(1), 115-129 (2002-10-25)
Preparative continuous annular chromatography, a method to separate proteins in a truly continuous manner, was investigated in an industrial environment. Plasma-derived clotting factor IX concentrate was used as model protein. Separation of vitronectin, a common impurity in commercial available factor
Masahiko Takanashi et al.
Journal of bioscience and bioengineering, 97(1), 78-81 (2005-10-20)
D(-)-3-hydroxybutyrate dehydrogenase (BDH; EC 1.1.1.30) from a poly(D(-)-3-hydroxybutyrate) (PHB) degrading bacterium, Acidovorax sp. SA1, was purified using Toyopearl DEAE-650M, red-Sepharose CL-4B, and Q Sepharose FF. The molecular mass of the enzyme was estimated as 27 kDa by SDS-PAGE and 110
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