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807465

TOYOPEARL® HW-65F Bulk Media

phase hydroxylated methacrylate, bottle of 500 mL, 30-60 μm particle size

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别名:
TOYOPEARL® HW-65 Size Exclusion Media
UNSPSC代码:
41115700

描述

HW-65F

产品线

TOYOPEARL®

形式

slurry

包装

bottle of 500 mL

参数

3 bar max. pressure

技术

HPLC: suitable

基质

hydroxylated methacrylic polymer

基质活性基团

hydroxylated methacrylate phase

粒径

30-60 μm

孔径

10,000-1,000,000 Da MW range

工作pH值

2-13

分离技术

size exclusion (SEC)

一般描述

Toyopearl HW-65 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 40,000-5,000,000 Da. It also is the base bead for all Toyopearl "-650" designated products.

应用

TOYOPEARL® media fractionate mixtures of proteins and other large molecular weight compounds over a wide size range. Applications include separating RNA from protein, resolving oligosaccharides by degree of polymerization, and isolating agglutinin while maintaining a high hemagglutination titer.

产品规格

Clean in place with 0.5 M NaOH or 0.1 M HCl.

外形

Shipped in 20% (v/v) ethanol.

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法律信息

Toyopearl is a registered trademark of Tosoh Corporation

象形图

Flame

警示用语:

Warning

危险声明

危险分类

Flam. Liq. 3

WGK

WGK 1

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

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Olga N Smol'kina et al.
Carbohydrate research, 347(1), 161-163 (2011-11-22)
A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was
Shu-fen Xu et al.
Fitoterapia, 83(1), 153-160 (2011-11-01)
Persimmon proanthocyanidin was fractionated on Toyopearl TSK-HW-50-F to yield a fraction with strong inhibition on the catalytic activity and edema-inducing activity and lethality of Chinese cobra PLA(2). Thiolysis suggested that the terminal units included C, EGCG and myricetin, and epicatechin
M V Pokrovskaya et al.
Protein expression and purification, 82(1), 150-154 (2012-01-10)
We have cloned ansB (YPTB1411) gene from Yersinia pseudotuberculosis Q66CJ2 and constructed stable inducible expression system that overproduce L-asparaginase from Y. pseudotuberculosis (YpA) in Escherichiacoli BL21 (DE3) cells. For purification of YpA we used Q-Sepharose and DEAE-Toyopearl column chromatography. We

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